Characterization by isoelectric focusing of pig heart aspartate aminotransferase
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چکیده
منابع مشابه
Isolation and purification of aspartate aminotransferase isoenzymes from human liver by chromatography and isoelectric focusing.
To purify cytoplasmic and mitochondrial isoenzymes of aspartate aminotransferase (EC 2.6.1.1) from human liver. We used heat treatment, ammonium sulfate precipitation, anion- and cation-exchange chromatography, affinity chromatography, and isoelectric focusing. Final preparations of the isoenzymes were homogeneous, with specific activities of 198 and 208 kU/g for the cytoplasmic and the mitocho...
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Isoelectric focusing on ultrathin polyacrylamide foils, coupled with isoenzymatic analysis using five different enzymes, was used to characterize blood-forms of T.b. brucei, T.b. rhodesiense and T.b. gambiense and procyclic forms of T.b. brucei and T.b. rhodesiense. The protein concentrations in the lysates were quantified. Qualitative as well as quantitative differences were found.
متن کاملAntigenic characterization of flavivirus structural proteins separated by isoelectric focusing.
Isoelectrofocusing of nonionic-detergent-disrupted flaviviruses separated the envelope glycoprotein of 53,000 to 58,000 daltons and the nucleocapsid protein of 14,000 daltons. The envelope protein and nucleocapsid protein were isolated at isoelectric points of pI 7.8 and 10.3, respectively. The antigenic determinants of St. Louis encephalitis, Japanese encephalitis, and dengue virus envelope an...
متن کاملKinetics and electrophoretic properties of the isozymes of aspartate aminotransferase from pig heart.
Cationic aspartate aminotransferase from pig heart was completely destroyed by heating to 75’ for 20 min in the purification procedure of Jenkins, Yphantis, and Sizer (11). Chromatography of the partially purified enzyme preparation on carboxymethyl cellulose according to the method of Henson and Cleland (8) yielded two enzyme fractions both of which migrated toward the anode upon starch gel el...
متن کاملThe binding of pyridoxal 5-phosphate to aspartate aminotransferase of pig heart.
1. Oestrone 3-glucuronide has been identified as the reaction product after incubation of oestrone with a rabbit-liver microsomal preparation, uridine diphosphate glucuronic acid being used as the glucuronic acid donor. The formation of this compound was studied under various experimental conditions. 2. The reaction showed a maximum at pH 8.08-2, and Km for oestrone was found to be 9-7 x 10-5M....
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1969
ISSN: 0014-5793
DOI: 10.1016/0014-5793(69)80354-6